TaqMan Real-Time Quantification of Epstein-Barr Virus in Severe Early Childhood Caries

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TaqMan Real-Time Quantification of Epstein-Barr Virus in Severe Early Childhood Caries

OBJECTIVES Early childhood caries (ECC) has several risk factors and it is important stressful/painful events of childhood and immunosuppression may occur during this unique rampant caries pattern. The changes in the host immune competence by compromised cellular immune system functions can activate Epstein Barr virus (EBV). The objective of this study was to determine whether the supragingival...

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بررسی توزیع گونه های انتخابی باکتری ساکن در نواحی مختلف حفره دهان در کودکان caries free و severe-early childhood caries به روش real time pcr

پوسیدگی دندانی یکی از شایع ترین بیماریهای مزمن دوران کودکی محسوب می شود. علیرغم کاهش جهانی پوسیدگی در دندانهای شیری، پوسیدگی زودرس دوران کودکی هنوز هم یک مشکل حاد در کشورهای در حال رشد و بعضی کشورهای رشد یافته محسوب می شود. پوسیدگی دندانی علت چندگانه دارد که از جمله می توان به سبک زندگی و سابقه ژنتیکی فرد اشاره نمود. فاکتورهای میزبان شامل بزاق و خصوصیات سطحی دندان همگی در بروز پوسیدگی دخیل هستن...

Comparison of Salivary Secretory IgA in Caries-free Children and Children with Severe Early Childhood Caries

Background & Aims: Sever early childhood caries (SECC) is a multifactor common disease that unfortunately there have been a lot of unknown points about it. This study was performed to compare the levels of total S-IgA in children with and without SECC. Methods: In this cross-sectional study 44 children aged 6-24 months were divided into two groups of with SECC (n=20) and without SECC (n=24). Th...

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Performance characteristics of two real-time PCR assays for the quantification of Epstein-Barr virus DNA.

We compared the performance of a laboratory-developed 5'-nuclease real-time polymerase chain reaction assay and a commercial assay (LightCycler, Roche Diagnostics, Indianapolis, IN) for quantification of Epstein-Barr virus (EBV) DNA. Using standards provided by the manufacturer, the LightCycler assay was linear from 100 to 1 million copies per reaction. Based on dilution of a plasmid containing...

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Comparison of commercial real-time PCR assays for quantification of Epstein-Barr virus DNA.

Clinical research suggests a role for viral load measurement in predicting and monitoring Epstein-Barr virus (EBV)-associated diseases. The aim of this study was to assess the performance of the recently commercially available quantitative assays for EBV based on real-time PCR: the RealArt EBV LC PCR kit and the LightCycler EBV quantification kit. A total of 87 samples were analyzed: 67 samples...

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ژورنال

عنوان ژورنال: European Journal of Dentistry

سال: 2010

ISSN: 1305-7456,1305-7464

DOI: 10.1055/s-0039-1697805